Proteomics Standards (sPRG)

Home

breadcrumbs-divide

Proteomics Standards (sPRG)

Proteomics Standards Research Group

Proteomics Standards Research Group (sPRG)

The mission of the ABRF Proteomics Standards Research Group (sPRG) is to promote and support the development and use of standards in proteomics for the benefit of all research laboratories, resource facilities and individual scientists including ABRF members and member laboratories.

Questions or interest in joining an ABRF research group?

The sPRG is committed to identifying and implementing technical standards that reflect the ABRF’s commitment to accuracy, clarity, and consistency in the field of proteomics. Examples of technical standards include, but are not limited to, reference materials, data sets, conditions and procedures that give proteomics researchers and analysts independent criteria to evaluate their abilities to produce predictable, consistent results. The sPRG strongly supports ongoing efforts for standardization of the recording and reporting of proteomics experiments.
Current Membership

Benjamin Neely - (Chair) National Institute of Standards and Technology
Susan Abbatiello, Waters Corporation
Josue Baeza - GlaxoSmithKline
Bhavin Patel - Thermo Fisher Scientific
Lindsay Pino - Talus Bio
Brian Searle - The Ohio State University
Magnus Palmblad  - Leiden University Medical Center
Sue Weintraub - (EB Liaison) - University of Texas Health Sciences Center at San Antonio

 
Studies

ABRF sPRG 2019 Study

(Closed). Validation and characterization results of the sPRG heavy-labeled phosphopeptide standard from the 2019 cross lab study were presented at the 2019 ABRF annual meeting in San Antonio (2019 poster). Manuscript forthcoming. Requests for access to raw data or participation in future studies should be directed to: sprg2016@googlegroups.com

ABRF sPRG 2018 Study

(Closed). The sPRG announces a new heavy-labeled phosphopeptide standard and a study to test your ability to detect endogenous phosphopeptides with it. The study is anonymous so everyone is encouraged to participate.

Study Results, Molecular & Cellular Proteomics, https://doi.org/10.1016/j.mcpro.2023.100639 

Study Announcement (110K)

Please see change:

data should be sent back to our bioshare: https://bioshare.bioinformatics.ucdavis.edu/bioshare/view/SPRG2018/

log in: abrfsprg2018@gmail.com (password stays the same as before)

    - 2018 sPRG Study Sample Instructions

    - 2018 sPRG report

    - 2018 phosphopeptide sequences

ABRF sPRG 2013 Study
- Study Announcement (110K)
- Data Results Template (136K)
- Study Sample Instructions (37K)
- Skyline Reporting Template
- Ion-trap Spectral Library
- Q-TOF/QQQ Spectral Library
- HCD Spectral Library
- Skyline Tutorial
- Raw Data Upload Instructions (651K)
- Human Swissport FASTA database (zipped)
- ABRF sPRG 2013 FASTA database (zipped) 
- Skyline Tutorial Data

ABRF sPRG 2011-2012 Study
- FASTA database file- zipped
- Sample Information Letter

ABRF sPRG 2010 Study
sPRG 2010 Study Sample Request (36K)

ABRF sPRG 2009 Study
ABRF sPRG Study 2009 Announcement

ABRF sPRG 2007 Study
ABRF sPRG Study 2007 Survey Open (14K)
ABRF sPRG Study 2007 Instructions
ABRF sPRG Study 2007 Announcement

ABRF sPRG 2006 Study
ABRF sPRG Study 2006 Announcement
ABRF sPRG Study 2006 Sample Letter
ABRF sPRG Study 2006 Data Request

Protocols
1) ABRF_sPRG_2011_2012_Study_Peptide_Information
2) The ABRF sPRG 2011 post translational modification standard peptide Spectral Library. The library was used by the team to produce both the presentation and poster. It is in NIST format compatible with MS Search and MSPepSearch.
Membership History
Member Name Organization Details
Philip Andrews Univ of Michigan Med School Member: 02/05 - 02/08
David Arnott Genentech, Inc. Member: 02/05 - 08/09
Christopher Ashwood Beth Israel Deaconess Medical Center Member through 11/21
Allis Chien Stanford University EB Liaison: 03/15 - 3/19
Christopher Colangelo Primary Ion Member: 08/09 - 03/12
Chair: 03/12 - 05/15
Craig Dufresne Thermo Fisher Scientific Member: 03/12 - 02/16
Jim Farmar Merck Member: 04/07 - 08/11
David Friedman ABRF Ad hoc: 03/12 - 06/12
EB Liaison: 05/10 - 03/12
Mary Ann Gawinowicz HHMI Columbia University Chair: 03/06 - 04/07
Member: 11/04 - 04/07
David Hawke UT MD Anderson Cancer Center  
Anthony Herren UC Davis Member: 05/17 - Chair: 03/19 - 03/21
Gordana Ivosev Sciex Member: 11/14 - 03/21
Alexander Ivanov Northeastern University Chair: 04/10 - 03/12
Member: 03/12 - 02/16
Christopher Kinsinger National Cancer Institute Member: 08/09 - 09/11
Antonius Koller Koch Institute at MIT Member: 03/12 - 03/21
Chair: 05/15 - 04/18
Jeffrey Kowalak NIMH Chair: 11/04 - 03/06
Member, EB Liaison: 11/04 - 03/10
William Lane Harvard University Member: 02/05 - 08/09
Kimberly Lee Cell Signaling Technologies Member: 04/17 - 03/21
Chair: 04/18 - 03/19
Ryan Leib Stanford University Member: 03/16 - 03/21
Kathryn Lilley University of Cambridge Member: 04/10 - 06/12
Member: 11/04 - 04/07
Rachel Loo UCLA Chair: 02/08 - 05/08
Larry Martin Northwestern University Member: 02/05 - 04/07
Karl Mechtler IMP Member: 04/07 - 03/12
Ad hoc: 03/12 - 06/12
Benjamin Neely National Institute of Standards and Technology Member: 10/19 -
Bhavin Patel Thermo Fisher Scientific Member: 03/16 -
Chair: 04/18 - 03/19
Brett Phinney Proteomics Core UC Davis Genome Center Member: 06/08 - 03/12, 03/16 - 03/19
Manfred Raida Proteomics.Asia Member: 04/07 - 04/10
Paul Rudnick NIST Ad hoc: 09/11 - 03/12
Brian Searle Ohio State University Member: 08/11 -
Chair: 04/18 - 03/19
Scott Shaffer University of Massachusetts Medical School Member: 02/10 - 02/16
Steve Stein NIST Member: 11/04 - 04/07
Susan Weintraub Univ. of Texas Health Science Center at San Antonio Member: 03/08 - 03/12
Ad hoc: 03/12 - 06/12
 
Publications
  1. sPRG 2009
    A Ivanov presentation, J Farmar presentation
  2. sPRG 2010
    A Ivanov, C Colangelo, J Farmar, C Kinsinger, J Kowalak, K Mechtler, B Phinney, M Raida, S Weintraub
    Development of a Phosphopeptide Standard for Proteomics
  3. sPRG 2011 - 2012
    AR Ivanov, CM Colangelo, CP Dufresne, J Farmar, DB Friedman, C Kinsinger, KS Lilley, K Mechtler, BS Phinney, KL Rose, SA Shaffer, ST Weintraub
    Development and characterization of a comprehensive standard for analysis of post‐translational modifications
  4. sPRG 2013
    AR Ivanov, CM Colangelo, CP Dufresne, DB Friedman, KS Lilley, K Mechtler, BS Phinney, KL Rose, PA Rudnick, BC Searle, SA Shaffer, ST Weintraub
    Interlaboratory studies and initiatives developing standards for proteomics