Proteomics Standards
Proteomics Standards (sPRG)
Mission
The mission of the ABRF Proteomics Standards Research Group (sPRG) is to promote and support the development and use of standards in proteomics for the benefit of all research laboratories, resource facilities and individual scientists including ABRF members and member laboratories.
Questions or interest in joining an ABRF research group? Contact us!
Current Membership
Benjamin Neely - (Chair) National Institute of Standards and Technology
Susan Abbatiello, Waters Corporation
Josue Baeza - GlaxoSmithKline
Bhavin Patel - Thermo Fisher Scientific
Lindsay Pino - Talus Bio
Brian Searle - The Ohio State University
Magnus Palmblad - Leiden University Medical Center
Sue Weintraub - (EB Liaison) - University of Texas Health Sciences Center at San Antonio
Studies
ABRF sPRG 2019 Study
(Closed). Validation and characterization results of the sPRG heavy-labeled phosphopeptide standard from the 2019 cross lab study were presented at the 2019 ABRF annual meeting in San Antonio (2019 poster). Manuscript forthcoming. Requests for access to raw data or participation in future studies should be directed to: sprg2016@googlegroups.com
ABRF sPRG 2018 Study
(Closed). The sPRG announces a new heavy-labeled phosphopeptide standard and a study to test your ability to detect endogenous phosphopeptides with it. The study is anonymous so everyone is encouraged to participate.
Study Results, Molecular & Cellular Proteomics, https://doi.org/10.1016/j.mcpro.2023.100639
Study Announcement (110K)
Please see change:
data should be sent back to our bioshare: https://bioshare.bioinformatics.ucdavis.edu/bioshare/view/SPRG2018/
log in: abrfsprg2018@gmail.com (password stays the same as before)
- 2018 sPRG Study Sample Instructions
- 2018 phosphopeptide sequences
ABRF sPRG 2013 Study
- Study Announcement (110K)
- Data Results Template (136K)
- Study Sample Instructions (37K)
- Skyline Reporting Template
- Ion-trap Spectral Library
- Q-TOF/QQQ Spectral Library
- HCD Spectral Library
- Skyline Tutorial
- Raw Data Upload Instructions (651K)
- Human Swissport FASTA database (zipped)
- ABRF sPRG 2013 FASTA database (zipped)
- Skyline Tutorial Data
ABRF sPRG 2011-2012 Study
- FASTA database file- zipped
- Sample Information Letter
ABRF sPRG 2010 Study
sPRG 2010 Study Sample Request (36K)
ABRF sPRG 2009 Study
ABRF sPRG Study 2009 Announcement
ABRF sPRG 2007 Study
ABRF sPRG Study 2007 Survey Open (14K)
ABRF sPRG Study 2007 Instructions
ABRF sPRG Study 2007 Announcement
ABRF sPRG 2006 Study
ABRF sPRG Study 2006 Announcement
ABRF sPRG Study 2006 Sample Letter
ABRF sPRG Study 2006 Data Request
Activities
ABRF 2018 Presentation on "sPRG: yet another phosphoproteomics standard?" by Brian Searle
ABRF 2017 Presentation on "sPRG: Development and Characterization of a Stable-Isotope Labeled Phosphopeptide Standard" by David Hawke/Toni Koller
ABRF 2016 Presentation on "sPRG: a Tale of Two Studies" by Brian Searle (same presentation with notes)
ABRF 2015 Presentation on "Further Development and Characterization of a Proteomics Standard Consisting of 1000 Stable Isotope Labeled Peptides"
by Gordana Ivosev
ABRF 2014 Presentation on "Development and Characterization of a Proteomics Standard Consisting of 1000 Stable Isotope Labeled Peptides"
Electronic Posters
sPRG 2019 poster presented at ABRF 2019
sprg_2019_poster.pdf
sPRG 2018 poster presented at ABRF 2018
sPRG 2017 poster presented at ABRF 2017
sPRG 2014 poster presented at ABRF 2014
sPRG 2013 poster presented at ABRF 2013
ABRF-sPRG 2011-2012 Study: Development and Characterization of a Comprehensive Standard for Analysis of Post-translational Modifications followed by Evaluation of the Standard by Study Participants
sPRG 2009 poster presented at ASMS 2009
ASMS08 sPRG 2009 Poster
ABRF sPRG Study 2007 Oral Presentation: Development and Evaluation of a Phosphoprotein Standard (PDF)
ABRF sPRG Study 2007 Poster (PDF)
ABRF sPRG Study 2006 Oral Presentation: A Proteomics Standard (PDF)
ABRF sPRG Study 2006 Poster: A Proteomics Standard (PDF)
Protocols
ABRF_sPRG_2011_2012_Study_Peptide_Information
The ABRF sPRG 2011 post translational modification standard peptide Spectral Library. The library was used by the team to produce both the presentation and poster. It is in NIST format compatible with MS Search and MSPepSearch.
Membership History
| Member Name | Organization | Participation |
|---|---|---|
| Philip Andrews | University of Michigan Medical School | Member: 02/05 - 02/08 |
| David Arnott | Genentech, Inc. | Member: 02/05 - 08/09 |
| Christopher Ashwood | Beth Israel Deaconess Medical Center | 02/08 - 11/21 |
| Allis Chien | Stanford University | EB liaison: 03/15 - 03/19 |
| Christopher Colangelo | Primary Ion | Member: 08/09 - 03/12 Chair: 03/12 - 05/15 |
| Craig Dufresne | Thermo Fisher Scientific | |
| Jim Farmar | Merck | Member: 04/07 - 08/11 |
| David Friedman | ABRF | Ad hoc: 03/12 - 06/12 EB liaison: 05/10 - 03/12 |
| Mary Ann Gawinowicz | HHMI Columbia University | Chair: 03/06 - 04/07 Member: 11/04 - 04/07 |
| David Hawke | UT MD Anderson Cancer Center | |
| Anthony Herren | UC Davis | Member: 05/17 - Chair: 03/19 - 03/21 |
| Gordana Ivosev | Sciex | Member: 11/14 - 03/21 |
| Alexander Ivanov | Northeastern University | Chair: 04/10 - 03/12 Member: 03/12 - 02/16 |
| Christopher Kinsinger | National Cancer Institute | Member: 08/09 - 09/11 |
| Antonius Koller | Koch Institute at MIT | Member: 03/12 - 03/21 Chair: 05/15 - 04/18 |
| Jeffrey Kowalak | NIMH | Chair: 11/04 - 03/06 Member, EB Liaison: 11/04 - 03/10 |
| William Lane | Harvard University | Member: 02/05 - 08/09 |
| Kimberly Lee | Cell Signaling Technologies | Member: 04/17 - 03/21 Chair: 04/18 - 03/19 |
| Ryan Leib | Stanford University | Member: 03/16 - 03/21 |
| Kathryn Lilley | University of Cambridge | Member: 04/10 - 06/12 Member: 11/04 - 04/07 |
| Rachel Loo | UCLA | Chair: 02/08 - 05/08 |
| Larry Martin | Northwestern University | Member: 02/05 - 04/07 |
| Karl Mechtler | IMP | Member: 04/07 - 03/12 Ad hoc: 03/12 - 06/12 |
| Benjamin Neely | National Institute of Standards and Technology | Member: 10/19 - |
| Bhavin Patel | Thermo Fisher Scientific | Member: 03/16 - Chair: 04/18 - 03/19 |
| Manfred Raida | Proteomics.Asia | Member: 04/07 - 04/10 |
| Paul Rudnick | NIST | Ad hoc: 09/11 - 03/12 |
| Brian Searle | Ohio State University | Member: 08/11 - Chair: 04/18 - 03/19 |
| Scott Shaffer | University of Massachusetts Medical School | Member: 02/10 - 02/16 |
| Steve Stein | NIST | Member: 11/04 - 04/07 |
| Susan Weintraub | Univ. of Texas Health Science Center at San Antonio | Member: 03/08 - 03/12 Ad hoc: 03/12 - 06/12 |