MOLECULAR INTERACTIONS RESEARCH GROUP 2002 STUDY

 

The mission of the Molecular Interactions Research Group (MIRG) is to:

  1. Teach how biophysical tools (such as isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), and analytical ultracentrifugation (AUC)) are used to quantitatively characterize biomolecular interactions in a resource facility environment
  2. Provide test systems to compare the capabilities of individual laboratories and across technologies
  3. Compile and publish the test system results in J. Biomolecular Techniques
  4. Organize and run workshops and other activities at annual ABRF meetings to accomplish this mission.

Additional information is at (www.abrf.org/index.cfm/group.show/MolecularInteractions.31.htm).

The MIRG 2002 Sudy

A major challenge in the molecular interactions field is to measure molecular-level binding parameters (affinity, stoichiometry, kinetics, and thermodynamics) that are both precise and accurate. This challenge is compounded by the desire to do this in high-throughput in order to keep up with the growing list of protein-protein and other biomolecular interactions.

In order to evaluate the capabilities of core facilities to characterize biomolecular interactions (variability in instrumentation, analytical methods, cross-technologies, etc.) the MIRG will distribute a protein-ligand pair (bovine carbonic anhydrase-II (CA-II) and 4-carboxy-benzensulfonamide (CBS)) to users of ITC, SPR, and AUC. The goals of the study are to: (1) compare the capabilities of individual laboratories, (2) identify areas that need improvement, and (3) define the extent to which two or more capabilities are available in one core or location.

Participants will be asked to measure one or more of the following: 1) affinity and apparent binding enthalpy change by ITC, 2) affinity and kinetics by SPR, and 3) molecular mass, homogeneity, and assembly state by AUC, using equilibrium and velocity approaches. Participating labs will be anonymous to the public and their results will be reported using numbers assigned when samples are sent.

How to Participate

Submit your name, E-mail, shipping address, and technology you will use (SPR, ITC or AUC).

For SPR, submit information to David Myszka (david.myszka@cores.utah.edu).

For ITC, submit information to Mike Doyle (Michael.Doyle@bms.com).

For AUC, submit information to Walter Stafford (stafford@bbri.org).

Samples and guidelines for participants will be sent to participants starting in January. Data from participants must be submitted by February 15, 2002.

Results from the study will be presented at ABRF 2002 and will be available thereafter on our web site and / or as a published article.