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Proteomics Standards Research Group (sPRG)

Mission

The mission of the ABRF Proteomics Standards Research Group (sPRG) is to promote and support the development and use of standards in proteomics for the benefit of all research laboratories, resource facilities and individual scientists including ABRF members and member laboratories.

The sPRG is committed to identifying and implementing technical standards that reflect the ABRF’s commitment to accuracy, clarity, and consistency in the field of proteomics. Examples of technical standards include, but are not limited to, reference materials, data sets, conditions and procedures that give proteomics researchers and analysts independent criteria to evaluate their abilities to produce predictable, consistent results. The sPRG strongly supports ongoing efforts for standardization of the recording and reporting of proteomics experiments.

Current Membership

Anthony W. Herren (Chair) - UC Davis
Kimberly A. Lee - Cell Signaling Technology
Brian C. Searle - Institute for Systems Biology/Proteome Software Inc.
Bhavin Patel - Thermo Fisher Scientific
Ryan Leib - Stanford University
Allis Chien - Stanford University
David Hawke - UT MD Anderson Cancer Center
Antonius Koller - Koch Institute at MIT
Gordana Isovev - Sciex
Christopher Colangelo (ad hoc) - Primary Ion 
Magnus Palmblad (EB Liaison) - Leiden University Medical Center

Studies

ABRF sPRG 2019 Study

(Closed). Validation and characterization results of the sPRG heavy-labeled phosphopeptide standard from the 2019 cross lab study were presented at the 2019 ABRF annual meeting in San Antonio (2019 poster). Manuscript forthcoming. Requests for access to raw data or participation in future studies should be directed to: sprg2016@googlegroups.com

ABRF sPRG 2018 Study

(Closed). The sPRG announces a new heavy-labeled phosphopeptide standard and a study to test your ability to detect endogenous phosphopeptides with it. The study is anonymous so everyone is encouraged to participate.

Study Announcement (110K)

Please see change:

data should be sent back to our bioshare: https://bioshare.bioinformatics.ucdavis.edu/bioshare/view/SPRG2018/

log in: abrfsprg2018@gmail.com (password stays the same as before)

    - 2018 sPRG Study Sample Instructions

    - 2018 sPRG report

    - 2018 phosphopeptide sequences

ABRF sPRG 2013 Study
    - Study Announcement 
(110K)
    - Data Results Template 
(136K)
    - Study Sample Instructions 
(37K)
    - Skyline Reporting Template 
    - Ion-trap Spectral Library 
    - Q-TOF/QQQ Spectral Library 
    - HCD Spectral Library 
    - Skyline Tutorial 
    - Raw Data Upload Instructions 
(651K)
    - Human Swissport FASTA database
(zipped) 
    - ABRF sPRG 2013 FASTA database
(zipped) 
    - Skyline Tutorial Data

ABRF sPRG 2011-2012 Study
    - FASTA database file- zipped 
    - Sample Information Letter

ABRF sPRG 2010 Study
sPRG 2010 Study Sample Request (36K)

ABRF sPRG 2009 Study
ABRF sPRG Study 2009 Announcement

ABRF sPRG 2007 Study
ABRF sPRG Study 2007 Survey Open (14K)
ABRF sPRG Study 2007 Instructions
ABRF sPRG Study 2007 Announcement

ABRF sPRG 2006 Study
ABRF sPRG Study 2006 Announcement
ABRF sPRG Study 2006 Sample Letter
ABRF sPRG Study 2006 Data Request

Protocols

1)ABRF_sPRG_2011_2012_Study_Peptide_Information
2)The ABRF sPRG 2011 post translational modification standard peptide Spectral Library. The library was used by the team to produce both the presentation and poster. It is in NIST format compatible with MS Search and MSPepSearch.

Membership History

Member NameOrganizationDetails
Philip AndrewsUniv of Michigan Med School Member: 02/05 - 02/08
David ArnottGenentech, Inc. Member: 02/05 - 08/09
Allis ChienStanford UniversityEB Liaison: 03/15 - 3/19
Christopher ColangeloPrimary Ion Member: 08/09 - 03/12
Chair: 03/12 - 05/15
Craig P. DufresneThermo Fisher ScientificMember: 03/12 - 02/16
Jim G. FarmarMerck Member: 04/07 - 08/11
David B. FriedmanABRF Ad hoc: 03/12 - 06/12
EB Liaison: 05/10 - 03/12
Mary Ann GawinowiczHHMI Columbia Univ Chair: 03/06 - 04/07
Member: 11/04 - 04/07
Anthony W. HerrenUC DavisMember: 05/17 -Chair: 03/19 -
Gordana IvosevSciexMember: 11/14 -
Alexander R. IvanovNortheastern University Chair: 04/10 - 03/12
Member: 03/12 - 02/16
Christopher R. Kinsinger   National Cancer Institute Member: 08/09 - 09/11
Antonius KollerColumbia UniversityMember: 03/12 -
Chair: 05/15 - 04/18
Jeffrey A. KowalakNIMH Chair: 11/04 - 03/06
Member, EB Liaison: 11/04 - 03/10
William S. LaneHarvard University Member: 02/05 - 08/09
Kimberly LeeCell Signaling TechnologiesMember: 04/17 -
Chair: 04/18 - 03/19
Ryan LeibStanford UniversityMember: 03/16 -
Kathryn S. LilleyUniversity of Cambridge Member: 04/10 - 06/12
Member: 11/04 - 04/07
Rachel LooUCLA Chair: 02/08 - 05/08
Larry MartinNorthwestern University Member: 02/05 - 04/07
Karl MechtlerIMP Member: 04/07 - 03/12
Ad hoc: 03/12 - 06/12
Bhavin PatelThermo Fisher ScientificMember: 03/16 -
Chair: 04/18 - 03/19
Brett S. PhinneyProteomics Core UC Davis Genome Center Member: 06/08 - 03/12, 03/16 - 03/19
Manfred R. RaidaProteomics.Asia Member: 04/07 - 04/10
Paul A. RudnickNIST Ad hoc: 09/11 - 03/12
Brian C. SearleProteome SoftwareMember: 08/11 -
Chair: 04/18 - 03/19
Scott A. ShafferUniversity of Massachusetts Medical SchoolMember: 02/10 - 02/16
Steve E. SteinNIST Member: 11/04 - 04/07
Susan T. WeintraubUniv. of Texas Health Science Center at San Antonio Member: 03/08 - 03/12
Ad hoc: 03/12 - 06/12

Publications

  1. sPRG 2009
  2. sPRG 2010
    A Ivanov, C Colangelo, J Farmar, C Kinsinger, J Kowalak, K Mechtler, B Phinney, M Raida, S Weintraub
  3. sPRG 2011 - 2012
    AR Ivanov, CM Colangelo, CP Dufresne, J Farmar, DB Friedman, C Kinsinger, KS Lilley, K Mechtler, BS Phinney, KL Rose, SA Shaffer, ST Weintraub
  4. sPRG 2013
    AR Ivanov, CM Colangelo, CP Dufresne, DB Friedman, KS Lilley, K Mechtler, BS Phinney, KL Rose, PA Rudnick, BC Searle, SA Shaffer, ST Weintraub
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