Many of the Members on the MMRG have been involved in microbiome analysis for over 20 years and have a strong appreciation for the field. They are dedicated and understand the needs of this new and exciting field that continues to grow at an exponential rate.
If you would like to be part of the MMRG, please contact Scott Tighe at email@example.com. Requirements of the research group are 1) Active membership in the ABRF, 2) copy of your CV, and 3) a letter of interest describing your interest, motivation, and how you can contribute.
The ABRF Metagenomics and Microbiome Research Group is composed of Core lab managers, bench scientists, and bioinformaticists. The goals of the MMRG will be to establish and evaluate techniques that core laboratory operators, directors, managers, and users commonly ask. Initial studies include assembling microbial standards and to characterize shortcomings of current metagenomic techniques. this includes optimizing DNA extraction protocols, library synthesis methods for different NGS platforms and developing best approaches for bioinformatics. The development of bacterial cocktails includes working with specific vendors in the field to establish a bacterial counting platform (similar to simple low cost mammalian cell counters) that can be used for low cost, high speed bacterial cell counting as needed for both research and clinical metagenomic quality control requirements. This enumeration device will be validated against standard techniques such as flow cytometry, Microscopic, and LSC techniques. Development of a fixation technique that preserves DNA and RNA without cell leakage is also required. We intend to work very closely with the appropriate vendor of the field to help advance this technology.
Scott Tighe (Chair) - Vermont Cancer Center
Ebrahim Afshinnekoo - Weill Cornell Medical College
Nadim Ajami - Baylor University
Don A. Baldwin - MicroPath ID Diagnostics
Nathan J. Bivens - University of Missouri
Russ Carmical - UTMB - Galveston
Caryn Evilia - Idaho State University
Stefan J. Green - University of Illinois at Chicago - DNAS Facility
Ian Herriott - University of Alaska Fairbanks
Jessica Hoffman – Vermont Cancer Center
Joshua R. Hyman –University of Wisconsin - Madison
Sarah Johnson - Georgetown University
Samantha Joye - University of Georgia
Jodie Lee (Ad hoc) - Qiagen
Shawn Levy - HudsonAlpha Institute for Biotechnology
Christopher E. Mason - Weill Cornell Medical College
Ken McGrath - Australian Genome Research Facility
Natalia G. Reyero Vinas - Mississippi State University
Rachid Ounit – University of California, Riverside
Dev Mittar – ATCC Microbiology Systems
Matthew L. Settles - University of Idaho
Alexander J. Spicer – Sheffield Hallam University
Kelley Thomas - Hubbard Center for Genome Studies
Christian Lytle - (EB Liaison) – Giesel School of Medicine at Dartmouth
The 2103 NARG study evaluated many DNA extraction techniques against specific bacteria. Results describe the efficiency of each technique.
Presentation from the 2014 ABRF Metagenomics session describing proper use of controls for metagenomics studies
Poster MGRG and XMP - Albany New York
2015 MGRG poster: Life at the extreme.