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Molecular Interactions Research Group (MIRG)

Mission

This Research Group has been retired.

A central goal of molecular biology is the determination of biomolecular function. This comes largely from a knowledge of the non-covalent interactions (both from a kinetic and thermodynamic point of view) that biological small and macro-molecules experience. Three technologies well suited for these studies are isothermal titration calorimetry, biosensors (including surface plasmon resonance) and analytical ultracentrifugation. These tools allow us to define protein-protein, protein-DNA and protein-small molecule interactions, to high resolution. Hence, a Molecular Interactions Research Group has been established to embrace these techniques, at first. Others may be added with time.

The mission of this Research Group is:

  1. To show how solution biophysical tools (ITC, biosensors, and AUC) are used to quantitatively characterize interactions of macromolecules with each other and with small molecules,
  2. To show how these tools work in a resource facility environment,
  3. To educate the ABRF members in the methodologies of the three core technologies,
  4. To provide test systems to be used to compare the capabilities of individual laboratories with each other, either within the three disciplines or among the disciplines,
  5. To compile the results of the analyses of these systems and publish the results in the Journal of Biomolecular Techniques or other appropriate publications, and
  6. To meet regularly to organize and run workshops or other activities at the annual ABRF meetings to accomplish this mission.

Studies

1)Initial results of the MIRG 2002 Study
    - View Document (4,965K)
2)Participate in the MIRG 2002 Study !
    - View Document 
3)Invitation letter for participation in the MIRG 2010 Study. The goal is a benchmarking surface plasmon resonance study (SPR) (or related biosensor technology) to evaluate the capabilities of different laboratories and instrumentation models to characterize competition and ternary interactions for three component protein interaction systems. To accomplish this, the MIRG has developed a standardized model three-component protein interaction system where two of the proteins (A+B) are known to bind to a third (C), and their binding mechanisms are also well understood.
    - MIRG 2010 Invitation Letter 
4)Slide presentation of MIRG2010 Benchmark Study: Molecular Interactions in a Three Component System
    - MIRG2010 Study Results (1,598K)
5)Slide presentation used as the introduction to the MIRG session at the ABRF2011 meeting in San Antonio TX
    - MIRG2011 session introduction (184K)
6)Summary of results for the MIRG 2011 Survey on Label-Free Biomolecular Interaction Technologies, conducted Jan-Feb 2011 and presented at the ABRF2011 meeting in San Antonio TX
    - MIRG2011 Survey Results (495K)
7)Invitation letter for participation in the MIRG 2012 Study.
    - View Document (72K)
8)Protocol and Report form for MIRG 2012 Study
    - View Document (144K)
9)Slide presentation at ABRF2013 of background for the MIRG 2012 Benchmark Study
    - View Document (788K)
10)Slide presentation at ABRF2013 of MIRG 2012 Benchmark Study results
    - View Document (1,961K)

Electronic Posters

Summary of results for the MIRG survey, conducted Dec 2007-Jan 2008, and presented at ABRF 2008.
    - View Document (224K)

Membership History

Recent:

Dr. Satya P. Yadav (Chair) - Cleveland Clinic Fndn Res Inst 
Michael Doyle - Bristol-Myers Squibb Pharma 
Mary Murphy - Reichert Inc. 
Dr Thomas Neubert - New York Univ Sch of Med 
Dr. Aaron P Yamniuk - Bristol-Myers Squibb 
Dr. Brett S Phinney (EB Liaison) - Proteomics Core UC Davis Genome Center

Member NameOrganizationDetails
Fumio ArisakaTokyo Institute of Technology Member: 06/01 - 04/07
Dr. Simon BergqvistPfizer Inc. Member: 01/08 - 03/14
Dr Olwyn ByronUniversity of Glasgow Member: 06/01 - 01/04
Michael DoyleBristol-Myers Squibb Pharma Chair: 09/07 - 12/07
Chair: 02/09 - 04/10
Chair: 01/99 - 04/03
Member: 04/03 - 01/05
EB Liaison: 03/06 - 09/07
Edward EisensteinCenter for Advanced Research in Biotechnology (CARB) Chair: 07/05 - 09/07
Member: 01/99 - 04/05
Edward EisensteinInstitute of Bioscience and Biotechnology Research Member: 11/07 - 01/08
Preston HensleyLotus Translational Medicine ChairEB Liason: 01/99 - 01/01
EB Liaison: 01/01 - 01/05
Dr. Christian R. LombardoCelgene-Signal Research Division Member: 06/01 - 06/05
David G. MyszkaUniversity of Utah Member: 01/99 - 04/07
Dr Thomas NeubertNew York Univ Sch of Med EB Liaison: 04/10 - 03/14
Dr. Andrew PattersonPenn State University Member: 02/14 - 02/14
Matthew K RobinsonFox Chase Cancer Center Member: 04/09 - 03/14
Dr. Frederick P SchwarzCARB/NIST Member: 06/01 - 02/09
Walter StaffordBoston Biomedical Research Inst. Member: 01/99 - 04/07
Jim A ThomsonPGRD La Jolla Chair: 04/03 - 04/05
Member: 04/05 - 09/07
Member: 01/01 - 04/03
Dr. Satya P. YadavCleveland Clinic Fndn Res Inst Member: 02/09 - 03/14
Member: 11/07 - 02/08
Dr. Aaron P YamniukBristol-Myers Squibb Chair: 04/10 - 03/14
Member: 01/09 - 04/10
Dr. Anthony T YeungFox Chase Cancer Ctr EB Liaison: 02/08 - 04/10

Publications

  1. ABRF MIRG 2002 study
    DG Myszka, YN Abdiche,a F. Arisaka, O Byron, E. Eisenstein, P Hensley, JA Thomson, CR Lombardo, F Schwarz, W Stafford, ML Doylej
  2. ABRF–MIRG Benchmark Study: Molecular Interactions in a Three-Component System
    AP Yamniuk,, SC Edavettal, S Bergqvist, SP Yadav, ML Doyle, K Calabrese, JF Parsons, E Eisenstein
  3. MIRG Survey 2011: Snapshot of Rapidly Evolving Label-Free Technologies Used for Characterizing Molecular Interactions
    SP Yadav,, S Bergqvist, ML Doyle, TA Neubert, AP Yamniuk
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