ABRF : DNA Sequencing Troubleshooting Resource

DSRG Sequencing Troubleshooting Web Resource

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New Problem Submission

Please complete the following form to submit your sequencing problem/solution to us. Provide as much detail as possible.

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Submitter Information
Information in this section will NOT be viewable by users. This info is only for the DSRG and may be used during the curation process.

Last Name
First Name
Email Address
Address

Problem Information
Problem Title
Problem Category
Problem Description
Trace or Image File
File Format
Trace or Image File 2
File Format (File 2)
Trace or Image File 3
File Format (File 3)
Problem Frequency
How Sporadic
Samples Affected
Problem Solution
Unsuccessful Solutions Attempted
Additional Comments

Instrument Details
Instrument Model
Capillary Length (cm)
Capillary Mfg
Polymer
Polymer Mfg
Gel Type
Gel Type Mfg
% Gel
Plate WTR (cm)
Plate WTR Mfg
Running Buffer
Running Buffer Mfg
Run Time (hr)
Temp (ºC)
Gel-loading
Run Module
Run Module Parameter 1
Run Module Modification 1
Run Module Parameter 2
Run Module Modification 2
Run Module Parameter 3
Run Module Modification 3
Run Module Parameter 4
Run Module Modification 4
Run Module Parameter 5
Run Module Modification 5
Analysis Software
Analysis Software Mfg
Basecaller
Basecaller Mfg
Quaity Assessment Software
Quaity Assessment Software Mfg

Sequencing Reaction
Template Type
Template Amount per reaction (ng)
Quantification Method
Purification Method
Agarose Gel Check
Primer Amount per reaction (pmol)
Primer Tm (ºC)
Primer Type
Standard Primer Type
Primer Purification
Reaction Volume (µl)
Sequence Chemistry
Dye Volume (µl)
Dilution Buffer
Dilution Buffer Volume (µl)
Dilution Buffer Mfg
Additive
Additive Type
Additive Concentration (mM)
Additive Mfg
Reaction Vessel

Thermal Cycling Information
Make
Model
Hot Start
Temp (ºC)
Number of Cycles
Denaturation Temp (ºC)
Denaturation Time (sec)
Annealing Temp (ºC)
Annealing Time (sec)
Extension Temp (ºC)
Extension Time (sec)

Post-sequencing Reaction
Reaction Clean-up
Clean-Up Mfg
Dried Down & Resuspended
Resuspension/Loading in
Resuspension/Loading in Volume
Resuspension/Loading in Mfg
Denaturation
Denaturation Temp (ºC)
Denaturation Time (sec)