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Submission Age Keyword(s)
Problem Category Instrument Model
Template Type Reaction Volume (µl)
Sequence Chemistry Dye Volume (µl)
Dilution Buffer Dilution Buffer Volume (µl)
Dilution Buffer Mfg
Additive Additive Concentration (mM)
Additive Type
Additive Mfg
Reaction Clean-up Resuspension/Loading in
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View ID Current Primary Sort Direction Indicator. Click to reverse sort direction.Submit. Date Updated on Title Category Instrument Template Type Seq. Chem.
Click here to open a detailed view of this record 132 14-Jul-08 15-Jul-08 low power, current and voltage Instrument Problem Amersham Biosci Alf/Alf Express PCR product N/A
Click here to open a detailed view of this record 131 27-Jun-08 17-Jul-08 get NNNNN sequence as result ! Please Help ! Applied Biosystems 3130/3130xl PCR product BigDye v3.1
Click here to open a detailed view of this record 130 18-Apr-08 28-May-08     Applied Biosystems 3100 PCR product N/A
Click here to open a detailed view of this record 128 27-Mar-08 17-Jun-08 Random, overlapping G peaks ! Please Help ! Applied Biosystems 3130/3130xl plasmid BigDye v3.1
Click here to open a detailed view of this record 114 13-Dec-07 14-Dec-07 Bad lots of polymer Instrument Problem Applied Biosystems 3130/3130xl double stranded DNA BigDye v3.1
Click here to open a detailed view of this record 112 12-Dec-07 23-May-08 Low A and G baseline Instrument Problem Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 111 07-Dec-07 13-Jun-08 extra A or T peaks ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 109 14-Nov-07 14-Nov-07 a very long read for comparison Other Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 104 24-Sep-07 14-Nov-07 Base dropout Sample Problem Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 100 20-Aug-07 23-Aug-07 Poly A problems ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v1.1
Click here to open a detailed view of this record 099 02-Aug-07 13-Aug-07 3130 Capillary regeneration Instrument Problem Applied Biosystems 3130/3130xl N/A BigDye v3.1
Click here to open a detailed view of this record 098 02-Aug-07 22-Aug-07 The Good, The Bad and  the Ugly- Short Sequence High signal Sample Problem Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 096 13-Jun-07 14-Jun-07 Adenovirus sequencing ! Please Help ! Applied Biosystems 3730/3730xl N/A BigDye v3.1
Click here to open a detailed view of this record 095 01-Jun-07 17-Oct-07 How many samples can be loaded per capillary? Software Problem Applied Biosystems 310 PCR product BigDye v3.1
Click here to open a detailed view of this record 094 18-Apr-07 23-May-08 Peak shouldering ! Please Help ! Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 093 29-Mar-07 14-Jun-07 homopolymeric regions ! Please Help ! Applied Biosystems 3130/3130xl genomic DNA BigDye v3.1
Click here to open a detailed view of this record 092 27-Mar-07 23-May-08 ski-slope effect ! Please Help ! Applied Biosystems 3100 PCR product BigDye v3.1
Click here to open a detailed view of this record 091 26-Mar-07 27-Mar-07 sequencing multiplex rxn ! Please Help ! Applied Biosystems 3130/3130xl PCR product BigDye v3.1
Click here to open a detailed view of this record 090 26-Mar-07 18-Apr-07 large peaks but no sequence ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 089 26-Mar-07 26-Mar-07 peak compression/overlap Sample Problem Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 088 13-Mar-07 15-Mar-07 Need Feedback on Heat Seals Other Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 085 26-Jan-07 17-Apr-08 Yellow haze Background Instrument Problem Applied Biosystems 3130/3130xl plasmid BigDye v3.1
Click here to open a detailed view of this record 084 24-Jan-07 31-Jan-07 N call Software Problem Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 083 24-Jan-07 04-Feb-07 SNPS? Sample Problem Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 082 04-Jan-07 23-May-08 Sequencing Reaction ! Please Help ! Beckman Coulter CEQ 8000 PCR product BigDye v3.1
Click here to open a detailed view of this record 079 05-Dec-06 11-Jan-07 Secondary PCR sequence ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 078 28-Nov-06 09-Apr-07 High background and low signals on G bases ! Please Help ! Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 072 30-Oct-06 02-Jul-07 late start, wide peaks, CsCl salt contamination Sample Problem Applied Biosystems 3730/3730xl plasmid BigDye v3.1
Click here to open a detailed view of this record 071 26-Oct-06 11-Jan-07 Sequence Gap –  SeqA analysis artifact Software Problem Applied Biosystems 3730/3730xl PCR product BigDye v3.1
Click here to open a detailed view of this record 070 26-Oct-06 30-Oct-06 General weak sequencing signal - filter clog Instrument Problem Applied Biosystems 3730/3730xl N/A BigDye v3.1
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