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Summaries from past ABRF Meetings and Workshops

These presentations were generously made available by participants at ABRF annual meetings. Most presentations consist of the slides or notes used by the speaker, converted to pdf file format. The files can be downloaded to your computer or viewed directly from your browser, depending on the configuration of your computer. More information on how you may view and/or download pdf files is available.

• Presentations from ABRF 2007
  
  
  • Posters
    
    • Poster contest winner: P6-T Tranche: Secure Decentralized Data Storage for the Proteomics Community Jason A. Falkner and P. C. Andrews
      University of Michigan, Ann Arbor, Michigan
      
      
    • Poster contest winner: P212-M High-Throughput Analysis of Protein Localization Dynamics by Mass Spectrometry Denise J. L. Tan¹, S. Hester¹, A. Martinez-Arias², and K. Lilley^{1
      1}Cambridge Centre for Proteomics and Cambridge University, Cambridge, United Kingdom, ²Department of Genetics, Cambridge University, Cambridge, United Kingdom.
      
      
    • Poster contest winner: P130-S Development and Evaluation of an Absolute qPCR Technique for Methicillin-Resistant Staphylococcus aureus (MRSA) in Ventilator Associated Pneumonia (VAP) by using Mini-bronchoalveolar Lavage (mini-BAL) Specimens
      Xue-Ping Wang¹, L. Goodwin¹, C. C. Ginnochio², S. Condon², P.Francois³,D. Ost^{4,5
      1}Feinstein Institute for Medical Research, North Shore-Long Island Jewish Health System, Manhasset, New York, ²Microbiology, Virology and Molecular Diagnostics Laboratory, North Shore-Long Island Jewish Health System, Lake Success, New York ³University Hospital of Geneva, CH-1211 Geneva 14, Switzerland, ^4,5Interventional Pulmonology, North Shore University Hospital, North Shore-Long Island Jewish Health System, Manhasset, New York.
      
      
    • Poster contest winner: P173-M Slippery when Translated: Extensive Programmed Ribosomal Frameshifting Revealed by Proteomics
      Rachel R. Ogorzalek Loo^1,3, Y.Yang², H. Mouttaki⁵, M. McInerney⁵, R. Gunsalus^3,4, and J. A. Loo^{1-3
      1}Department of Biological Chemistry, ²Department of Chemistry & Biochemistry, ³Molecular Biology Institute, ⁴Department of Microbiology, Immunology, and Molecular Genetics, University of California-Los Angeles ⁵Department of Botany & Microbiology, University of Oklahoma, Norman, OK
      
      
  • Research Group Presentations
    
    
    • DNA Sequencing Research Group
      
      
      • 2007 Pilot Study: A Comparison of Variant Screening Strategies - DNA Sequencing and SNaPshot
      
      
    • Edman Sequencing Research Group
      
      
      • ESRG 2007 Poster: Procedures used to Determine the N-Terminal Sequence of a Blocked Protein
      • ESRG 2007 Oral Presentation: Procedures used to Determine the N-Terminal Sequence of a Blocked Protein
      
      
    • Microarray Research Group
      
      
      • MARG 2007 Research Poster: Comparison of Comparative Genomic Hybridization Technologies Across Microarray Platforms
      • MARG 2007 Educational Session: Microarray Experiment Design and Data Interpretation
      • MARG 2007 Research Presentation: Comparison of Comparative Genomic Hybridization Technologies Across Microarray Platforms
      
      
    • Nucleic Acids Research Group
      
      
      • NARG 2007 Real-Time PCR Survey Presentation
      • NARG 2007 Real-Time PCR Survey Poster
      • NARG 2007 Real-Time PCR Survey Questions
      • NARG 2007 Real-Time PCR Survey Raw Data
      
      
    • Protein Expression Research Group
      
      
      • PERG Survey 2007: Bottlenecks in protein expression
      
      
    • Proteome Informatics Research Group
      
      
      • sPRG-BIC 2007 Study Poster: Bioinformatic Evaluation of Datasets Derived from the ABRF sPRG Proteomics Standard
      • sPRG-BIC 2007 Study Presentation: Bioinformatics Committee Exploration of the sPRG2006 Study Data Sets
      
      
    • Proteomics Research Group
      
      
      • ABRF PRG 2007 Poster: Advanced Quantitative Proteomics Study
      • ABRF PRG 2007 Presentation: Advanced Quantitative Proteomics

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    ABRF '06: Integrating Science, Tools and Technologies with Systems Biology, February 11-14, 2006, Long Beach California.
    
    
    • Archived meeting information from FASEB site.
      
      
    • ABRF2006 meeting abstracts from the JBT (March 06).
      
      
    • Abstracts from Invited Speakers, Tutorials, Research Presentations and Posters.
      
      
    • Daily Detailed Schedule showing all presentations
      
      
    • Presentations from ABRF 2006
      
      
      • ABRF 2006 Satellite Meeting
        
        
        • DNA Sequencing Core DNA Sequencing Core Workflow & Workflow & Customer Interaction (716KB) Helaman Escobar, DNA Sequencing & Genomics Core Facility. University of Utah, Salt Lake City, UT, United States.
        • Implementing a Commercial LIMS into a Medium-Throughput DNA Sequencing Facility (1.3 MB) Debbie Adam, University of British Columbia, Vancouver, British Columbia, Canada.
          
        • In-house & Commercial LIMS: 4D, Finch (380 KB) Jan Kieleczawa, Wyeth Research/ BT, Cambridge, MA, United States.
        • Comparison of Different LIMS (32 KB)
          
        • Failure Mode Analysis - HT Pipeline (766 KB) George Yang, B.C. Genome Sciences Centre, Vancouver, British Columbia, Canada.
          
        • Optimizing the Small Core Laboratory "Tips and Tweaks" (180 KB) Michelle Detwiler, Roswell Park Cancer Institute, Buffalo, NY, United States.
          
        • Improved Protocols for Bisulfite Sequencing and Fragment Analysis of Methylated gDNA (435 KB) Dr. Achim Karger, Applied Biosytems, Foster City, CA, United States.
          
        • Confirmation Sensitive Capillary Electrophoresis (CSCE) (676 KB) Chris Mattocks, National Genetics Reference Laboratory, Wessex-Salisbury, England.
          
        • Ecotilling: High-throughput discovery of variation in DNA (4.3MB) Luca Comai, University of Washington, Seattle, WA, United States.
          
        • High-throughput BAC Fingerprinting For Constructing Physical Maps (1.2 MB) Ming-Cheng Luo, University of California- Davis, Davis, CA, United States.
        • DNA Footprinting (2.4 MB) DNA Footprinting with an automated DNA Analyzer: does the shoe fit? Michael Zianni, Ohio State University, Columbus, OH, United States.
          
          
      • Tutorials
        • High Throughput Methods for SNP Genotyping.
          Session Organizer: Karl Hager, Yale University, Keck Biotechnology Lab, New Haven, CT, United States.
          
          • SNP Genotyping at HPCGG (2.6 MB)
            Alison Brown: Harvard Partners Center for Genetics and Genomics, Cambridge, MA, United States.
            Utilization of Low Cost Multiplex Single Nucleotide Polymorphism (SNP) Genotyping Techniques.(570 KB)
            Robert Welch, NCI Core Genotyping Facility, Gaithersburg, MD, United States.
            
            
      • Research Group Presentations
        
        
        • DNA Sequencing Research Group
          
          • 2006 General Survey of DNA Sequencing Facilities (212 KB)
            Debbie Adam, University of British Columbia, Vancouver, British Columbia, Canada.
            
          • DSRG Launch of the Web-based Trouble-Shooting Resource (756 KB)
            Peter A. Schweitzer, Cornell University, Ithaca, NY, United States
            
            
        • Fragment Analysis Research Group
          
          • FARG 2006 SURVEY: A Comparative Study of Methodology and Instrumentation Uses of Laboratories Performing DNA Fragment Analysis (212 KB)
            Chris Lytle, Dartmouth College, Hanover, NH, United States
            Katia Sol-Church, Nemours Children's Clinic, Wilmington, DE United States.
            
            
        • Nucleic Acids Research Group
          
          • Nucleic Acids Research Group Study: Priming Strategies for Real-Time RT-PCR (9.5 MB)
            Deborah Grove, Penn State University, University Park, PA, United States.
          • Multiplex Real-Time qPCR (1.3 MB)
            Tim Hunter, University of Vermont, Burlington, VT, United States.
            
            
      • Posters
        • Poster R7-S FARG 2006 Survey-A Comparative Study of Methodology and Instrumentation Uses in Laboratories Performing DNA Fragment Analysis and Genotyping K. Sol-Church¹, D. Bintzler², J. Forrester³, R. Keefe⁴, D. Kristt⁵, C. Lytle⁶; ¹Nemours Children's Clinic, Wilmington, DE, United States, ²University of Cincinnati, Cincinnati, OH, United States, ³University of Missouri, Columbia, MO, United States, ⁴Wadsworth Center/NYS DOH, Troy, NY, United States, ⁵Rabin Medical Center, Petah Tikva, Israel, ⁶Dartmouth College, Hanover, NH, United States.
          
        • Poster R9-T Nucleic Acids Research Group Study: Priming Strategies for Real-Time RT-PCR D. S. Grove¹, P. S. Adams², Y. Bao³, S. Bustin⁴, T. Hunter⁵, G. Shipley⁶, A. Yeung⁷; ¹Penn State University, University Park, PA, United States, ²Trudeau Institute, Saranac Lake, NY, United States, ³University of Virginia, Charlottesville, VA, United States, ⁴Queen Mary University of London, London, United Kingdom, ⁵University of Vermont, Burlington, VT, United States, ⁶UT Health Science Center-Houston, Houston, TX, United States, ⁷Fox Chase Cancer Institute, Philadelphia, PA, United States.
          
        • Poster contest winner: Poster P16-S A Comprehensive Large-Scale Evaluation of Gene Expression Measurements Across Different Hybridization-Based Technologies.
          Winston P. Kuo1, F. Liu², T. Kristain-Jenssen³, E. Hovig², G. Short¹, S. Perrin⁴, R. Bumgarner⁵, R. Jenssen⁶, P. Park¹, L. Ohno-Machado¹, C. Cepko^{1
          1}Harvard Medical School, Boston, Massachusetts, ²The Norwegian Radium Hospital, Olso, Norway, ³PubGene, Olso, Norway, ⁴Biogen Idec, Boston, Massachusetts, ⁵University of Washington, Seattle, Washington, ⁶University of Massachusetts Boston, Boston, Massachusetts
          
        • Poster contest winner: Poster P49-S Characterization of GlnK and GlnB Uridylylation in Response to Nitrogen Availability for Rhodopseudomonas palustris.
          Heather M. Connelly, D. A. Pelletier, T. Yuan, P. K. Lankford, R. L. Hettich;
          Oak Ridge National Laboratory, Oak Ridge, Tennessee
          
        • Poster P81-T DNA Sequencing Research Group-2006 General Survey of DNA Sequencing Facilities D. Adam¹, J. Kieleczawa², H. Escobar³, M. Detwiler⁴, R. Pershad⁵, D. S. Needleman⁶, P. Schweitzer⁷, G. J. Wiebe⁸, S. Hardin⁹; ¹University of British Columbia, Vancouver, BC, Canada, ²Wyeth Research, Cambridge, MA, United States, ³University of Utah, Salt Lake City, UT, United States, ⁴Roswell Park Cancer Institute, Buffalo, NY, United States, ⁵University of Texas M.D. Anderson Cancer Center, Houston, TX, United States, ⁶USDA ARS Eastern Regional Research Center, Wyndmoor, PA, United States, ⁷Cornell University, Ithaca, NY, United States, ⁸Max Planck Institute of Molecular Biology & Genetics, Dresden, Germany, ⁹University of Houston, Houston, TX, United States.
          
        • Poster contest winner: Poster P84-T Evaluating Mixed DNA Samples: Comparison of STR-PCR and SNP-Pyrosequencing for Forensic and Biomedical Monitoring. Deborah L. Stabley¹, A. Richez¹, D. Kristt², Katia Sol-Church¹¹Nemours Children's Clinic, Wilmington, Delaware, ²Rabin Medical Center, Petah Tikva, Israel
          
        • Poster contest winner: Poster P175-S Phosphoproteomic Characterization of Human Eosinophils Activated with GM-CSF.
          Travis W. Young, K. Pazdrak, J. Wiktorowicz, S. Stafford, A. Kurosky University of Texas Medical Branch, Galveston, Texas
          

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    ABRF '05: BioMolecular Technologies: Discovery to Hypothesis, February 5-8, 2005, Savannah, GA.
    
    

    • Archived meeting information from FASEB site.
      
      
    • ABRF2005 meeting abstracts reprinted with permission of the JBT.
      • Schedule and All Abstracts from Invited Speakers, Research Group Poster/Presentation and Posters.
        (471 KB) Hint - use search to help find what you want.
        
        
      • Daily Detailed Schedule showing all presentations
        
        
    • Presentations from ABRF 2005
      
      
      • Scientific Session Presentations
        • S1 Next Generation DNA Sequencing Technologies.
          Chair: Susan Hardin; VisiGen Biotechnologies, Inc., Houston, TX, United States.
          
          
        • • Real-Time DNA Sequencing (2.25 MB)
            S. Hardin; VisiGen Biotechnologies, Inc., Houston, TX, United States.
            
            
        • S11 Genetic Analysis: Study Design, Methodology,and Statistical Considerations.
          Chair: Katia Sol-Church (Session Chair), The Alfred I duPont Hospital for Children, Wilmington, DE, United States.
          
          
          • Study Design and SNP Selection Strategies for Genetic Association Studies
            Trevor Woodage, Applied Biosystems, Rockville, MD, United States.
            
            
          • No More Gels: Alternative Genotyping Platforms (3.7 MB)
            Cynthia Spittle, Chase Cancer Center, Philadelphia, PA, United States.
            
            
          • Statistical Tools for Disease Gene Mapping and Association Studies (642 K)
            Marcella Devoto, The Alfred I duPont Hospital for Children, Wilmington, DE, United States.
            
            
      • Tutorials
        • T3 Benchmarking to Improve Lab Operations.
          Chair: Ronald L. Niece, Research Resources & Technologies, Tuston, CA, United States.
          
          
          
          • Benchmarking to Improve Lab Operations - Taking It to the Next Level (225 KB)
            Lyn Faas, Seattle Public Utilities, Seattle, WA, United States.
            
            
        • T4 Comparative Protein Structure Modeling.
          Chair: Rolf Apweiler: EMBL-EBI, Hinxton, Cambridgeshire, United Kingdom.
          
          
          • Sequence to Structure Alignment Problem in Comparative Modeling (225 KB)
            Andras Fiser: Albert Einstein College of Medicine, Bronx, NY, United States.
            
            
          • Molecular Modeling as a Biological Resource Facility. (6.5 MB)
            Roland Dunbrack, Fox Chase Cancer Center, Philadelphia, PA, United States.
            
            
          • T10 Utilizing qPCR to Validate Microarray Results.
            Chair: Gregory Shipley: University of Texas Health Science Center, Houston, TX, United States.
            
            
            • Validation of Microarray Data via Quantitative Real-Time PCR (2.2 MB)
              Tim Hunter: University of Vermont, Burlington, VT, United States.
              
              
        • High-Throughput Real Time PCR Approaches for Microarray Sample Assessment and Target Validation.
          Andrew I. Brooks, University of Rochester Medical Center, Rochester, NY, United States.
          
          
        • Round Table Presentations
          • RT3 A New Method and Solutions for an Old Problem in Quantitative PCR.
            Chair: Deborah Grove; Penn State University, University Park, PA, United States.
            
            
            • LATE-PCR and Allied Technologies: Novel Real-time Methods and Reagents for Rapid, Reliable Genetic analysis Down to the Level of Single Cells. (5.2 MB)
              Lawrence Wangh
              Brandeis University, Waltham, MA, United States.
              
              
            • Making Biological Sense of QPCR Data from Small Samples - Process Considerations of Sample Preparation to Normalized Gene Expression Profiling. (1.2 MB)
              Rheinhold Mueller: Stratagene Europe, Amsterdam, The Netherlands.
              
              
          • RT4 Biomarker Discovery: Have We Learned How to Do This Yet?
            Chair: Stephen A. Carr; Broad Institute of MIT and Harvard, Cambridge, MA, United States.
            
            
            • Stephen A. Carr, Michael Gillette (1.5 MB)
              Broad Institute of Harvard and MIT, Cambridge, MA, United States.
              
              
            • Kevin Coombes (81 KB)
              University of Texas, MD Anderson Cancer Center, Houston, TX, United States.
              
              
            • Kojo Elenitoba-Johnson (20 KB)
              University of Utah School of Medicine, Salt Lake, UT, United States.
              
              
            • Gregory J. Opiteck (327 KB)
              Caprion Pharmaceuticals, Inc., Montreal, QC, Canada.
              
              
            • Sushmita Mimi Roy (1.0 MB)
              SurroMed Inc., Menlo Park, CA. United States.
              
              
            • Leigh Anderson
              Plasma Proteome Institute, Washington, DC, United States.
              
              
          • RT6 Strategies for SNP Genotyping in Core Labs (57 KB)
            
            
            • Comparison of Four SNP Genotyping Technologies: Taqman Allelic Discrimination, Pyrosequencing, Sequenom MassARRAY MALDI-TOF, ABI SNPlex (412 KB)
              Karl Hager W. M. Keck Foundation Biotechnology Resource Laboratory Yale University, New Haven, CT, United States.
              
              
            • SNP Genotyping at HPCGG (2.9 MB)
              Alison Brown Partners Healthcare System Harvard Medical School, Cambridge, MA, United States.
              
              
            • Evaluating Costs for Two SNP Platforms (1.1 MB)
              Peter Schweitzer, Biotechnology Resource Center, Cornell University, Ithaca, NY, United States.
              
              
        • Research Group Presentations
          • Nucleic Acids Research Group
            NARG 2005 Research Group presentation (1.3 MB)
            Scottie Adams. Trudeau Institute, Saranac Lake, NY, United States.
            Brian Holloway, CDC, Atlanta, GA, United States.
            Greg Shipley, University of Texas Health Science Center, Houston, TX, United States.
            Stephen A. Bustin, University of London, London, England, United Kingdom
            
            
        • Posters
          • Poster R1-S Nucleic Acid Research Group Study: A Comparison of Real-Time RT-PCR Technique, Chemistries, and Hardware in Laboratories Utilizing the Same Assay. GL Shipley¹ PS Adams², Y Bao³,SA Bustin⁴ DS Grove⁵,BP Holloway⁶, and AT Yeung⁷; ¹University of Texas Health Science Center, Houston, TX, United States,²Trudeau Institute, Saranac Lake, NY, United States, ³University of Virginia, Charlottesville, VA, United States, ⁴ University of London, London, England, United Kingdom⁵ Pennsylvania State University, University Park, PA, United States, ⁶CDC, Atlanta, GA, United States, ⁷ Fox Chase Cancer Center, Philadelphia, PA, United States. (1.6 MB bookmarked)
            
            
          • Poster R8-M DSRG 2005 - DNA Sequencing Troubleshooting Guide. Heather Lin¹, Debbie Adam², Helaman Escobar³, David S. Needleman⁴, Kevin L. Knudtson⁵, Rashmi Pershad⁶, Peter A. Schweitzer⁷,Ted Thannhauser⁸and Glenis J. Wiebe⁹. ¹DigiSapien, Redmond, WA, ²University of British Columbia, Vancouver, BC, ³University of Utah, Salt Lake City, UT, ⁴USDA ARS Eastern Regional Research Center, Wyndmoor, PA, ⁵University of Iowa, Iowa City, IA, ⁶University of Texas M.D. Anderson Cancer Center, Houston, TX, ⁷Cornell University, Ithaca, NY, ⁸USDA-ARS, Ithaca NY, ⁹Max Planck Institute of Molecular Cell Biology & Genetics, Dresden, Germany. (396 KB)
            
            
          • Poster contest winner: P29-M Mass Spectrometric Investigation of Ribosome Gymnastics. CE Doneanu¹, D Lindsley², P Bonthuis², VD Nguyen², and JA Gallant²; ¹Department of Medicinal Chemistry, ² Department of Genome Sciences, University of Washington, Seattle, WA, United States. (108 KB)
            
            
          • Poster contest winner: P143-M Approaches and Tools for Biomarker Discovery via Intact Protein Profiling. TL Williams, SM Musser; U.S. Food and Drug Administration, College Park, MD, United States. (2.1 MB)
            
            
          • Poster contest winner: P167-T Proteomics of the Microbial Community in an Acid Mine Drainage Biofilm. Nathan VerBerkmoes¹, R. J. Ram², M. P. Thelen², G. W. Tyson², B. J. Baker², J. F. Banfield², R. L. Hettich¹. ¹Oak Ridge National Laboratory, Oak Ridge, Tennessee, ²University of California, Berkeley, Berkeley, California, United States. (2.5 MB)
            
            
          • Poster contest winner: P205-S Differential Protein and Neuropeptide Expression in Experimental Parkinson's Disease. P. E. Andren¹, K. Skold¹, M. Svensson¹, A. Nilsson¹, M. Falth¹, P. Svenningsson²; ¹Uppsala University, Uppsala, Sweden, ²Karolinska Institute, Stockholm, Sweden. (568 KB)
            
            
          • Poster contest finalist: P203-M Differential Protein Expression in Brassica oleracia: Changes Associated with the Ability to Accumulate High Concentrations of Carotinoids. T. W. Thannhauser, Y. Yang, B. Xiang, S. Lu, L. Li; USDA-ARS, Ithaca, NY, United States. (819 KB)
            
            
          • Poster P31-S Mass Spectrometry Analysis of Sex-based Individual Venom Variation Among 18 Bothrops jararaca Sibling Snakes. Serrano, S.M.T. ¹, Furtado, M.F. ², Konno, K. ¹, Camargo, A.C.M. ¹, Pimenta, D.C. ^{1 1}Laboratório Especial de Toxinologia Aplicada/CAT-CEPID, ²Laboratório de Herpetologia, Instituto Butantan, 05503-900, Sao Paulo-SP, Brazil. (1459 KB)
            
            
          • Poster P53-M Faster PCR Amplification Using the Applied Biosystems 9800 Thermal Cycler. Matthew Nix, Pei Li Chong, Alicia Yang, Vicki Pandey, Paul Kotturi, and Damien Luk. Applied Biosystems, Foster City, CA, United States. (2.4 MB)
            
            
          • Poster P86-M Mitochondrial DNA Data Analysis Using SeqScape Software v2.5. A. Pradhan, Q. Doan, J. Sorenson, R. Fang, K. Poulter, M. Rydland, C. Forbes, S. Vijaychander, R. Nutter, S. Nidtha, P.Baybayan. Applied Biosystems, Foster City, CA, United States. (2.4 MB)
            
            
          • Poster P91-S VariantSEQr Resequencing System Process for the Discovery of Human Gene Variants. ¹Diane Bond, ¹Peter Ma, ¹Rixun Fang, ¹Primo Baybayan, ¹Soumya Nidtha, ¹Lilley Leong, ¹Xiaohui Chen, ²Mary Ann Rydland, ²Xiaoying Lin, ¹Karen Poulter, ¹Jon Sorenson and ¹Cheryl Heiner. ¹Applied Biosystems, Foster City, CA, United States, ²Celera Genomics, Rockville, MD, United States. (1.8 MB)
            
            
          • Poster P108-T Increased Productivity Using One Polymer and One ArrayLength for Multiple Applications. Take Ogawa, Steve Berosik, Larry Joe, Allen Swei, Jim Bourey, Jim Burrows, Suresh Pisharody, Lauren Seilnacht, Sreeram Santhanam and Ruben Pingue. Applied Biosystems, Foster City, CA, United States. (524 KB)
            
            
          • Poster P109-S DNA Resequencing and Variant Identification of the CCL24 Gene. Denise M. Schmidt, Jennifer D. Tan, George A. Fry, Primo A. Baybayan, Quynh C. Doan, Anjali A. Pradhan, Soumya S. Nidtha, and Thomas J. McElroy. Applied Biosystems, Foster City, CA, United States. (986 KB)
            
            
          • Poster P110-M BAC Fingerprinting Application on Applied Biosystems 3730/3730xl DNA Analyzers. Alicia Yang and Charles Reece. Applied Biosystems, Foster City, CA, United States. (1.3 MB)
            
            
          • Poster P111-T Using a High-Performance Capillary Electrophoresis System for Both Fragment Analysis and Sequencing Applications. Jennifer D. Tan, Denise M. Schmidt, Surekha Karudapuram, Allen N. Swei, George A. Fry, Larry K. Joe, Ruben A. Pingue, and Thomas J. McElroy. Applied Biosystems, Foster City, CA, United States. (609 KB)
            
            
          • Poster P112-S Use of a Single System for Multiple Fragment Analysis Applications. Surekha Karudapuram, Larry Joe, Ariana Wheaton, Yuandan Lou and Thomas McElroy. Applied Biosystems, Foster City, CA, United States. (536 KB)
            
            
          • Poster P130-S A Rapid On-Membrane Digestion Protocol for the Full-length Sequencing of Monoclonal Antibodies V. C. Pham, K. Schmidt, W. Sandoval, I. Mohtashemi, A. Izrael-Tomasevic, L. Phu, W. Henzel, D. Arnott, J. Lill. Genentech, South San Francisco, CA, United States. (96 KB)
            
            
          • Poster P159-S From Gel to MS: Factors Influencing Peptide Recovery from Gels. S. Francis-McIntyre, D. Sugden, I. Riba, S. J. Gaskell, The University of Manchester, Manchester, United Kingdom. (545KB)
            
            

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      ABRF '04: Integrating Technologies in Proteomics and Genomics, February 28-March 2, 2004, Portland, Oregon.
      
      
      • Archived meeting information from FASEB site
        
        
      • ABRF2004 meeting abstracts reprinted with permission of the JBT.
        
        
    • • Abstracts from Invited Speakers and Research Group Poster/Presentation Abstracts
        
        
      • Poster Abstracts
        
        
      • Presentations from ABRF 2004
        
        

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      ABRF '03: Translating Biology Using Proteomics and Functional Genomics, February 10-13, 2003, Denver, Colorado.
      
      
      • Archived meeting information from FASEB site
        
        
      • Abstracts Reprinted with permission of the JBT.
        
        
      • Presentations from ABRF 2003
        
        
        • Tutorials
          • Tutorial Session 4: Real - Time PCR
            Chair: Pamela Scott Adams, Trudeau Institute, Saranac Lake, NY.
            
            Single Nucleotide Polymorphism Genotyping: Why Use Real-Time PCR and Considerations For Facility Set Up.
            S. Bayliss ; Wellcome Trust Clinical Research Facility, Edinburgh, United Kingdom.
            
            
        • Posters
          • Poster P23-T Quantitative Determination of Non-covalent Protein-Ligand Interactions Using Automated Nanoelectrospray Mass Spectrometry. S. Zhang¹, C.K. Van Pelt¹ and G. Williams²; ¹Advion BioSciences, Inc., Ithaca, NY,USA, ²Advion BioSciences, Ltd., Norwich, UK.
            
            
          • Poster P51-W Identification of Phosphorylated and O-Glycosylated Sites in Peptides by Chemically-Targeted Proteolysis (CTID).G. M. Hathaway, J. Zhou, F. Rusnak; Caltech, Pasadena, CA, United States.
            
            
          • Poster P56-T Identification of the CRALBP Ligand Binding Pocket by Photoaffinity Labeling. Z. Wu^1,2, A. Hasan¹, K. Nakanishi³, J. W. Crabb^1,2; ¹Cleveland Clinic Foundation, Cleveland, OH, United States, ²Cleveland State University, Cleveland, OH, United States, ³Columbia University, New York, NY, United States.
            
            
          • Poster P111-W Precipitation of Large, High-Abundance Proteins from Serum with Organic Solvents. A. J. Alpert¹, A. K. Shukla²; ¹PolyLC Inc., Columbia, MD, United States, ²Glygen Corp., Columbia, MD, United States.
            
            
          • Poster P137-T Characterization of Lipid Oxidation Products and Proteins in Bruch's Membrane from Normal and AMD Donor Eyes. X. Gu¹, K. Shadrach¹, M. Sun², K. A. West¹, L. Shan³, S. L. Hazen³, R. G. Salomon², J. G. Hollyfield¹, J. W. Crabb¹; ¹Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, OH, United States, ²Chemistry Department, Case Western Reserve University, Cleveland, OH, United States, ³Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, United States.
            
            
          • Poster P138-W Identification of Visual Cycle Protein-Protein Interactions. S. K. Bhattacharya¹, Z. Wu¹, K. A. West¹, Z. Jin¹, M. Nawrot², J. C. Saari², J. W. Crabb¹; ¹Cleveland Clinic Foundation, Cleveland, OH, United States, ²University of Washington, Seattle, WA, United States.
            
            
          • Poster P170-Th Human Retinal Pigment Epithelium Protein Database. K. A. West^1,2, J. Sun^1,2, L. Yan³, K. Shadrach^1,2, A. Hasan^1,2, M. Miyagi⁴, J. S. Crabb^1,2, J. G. Hollyfield^1,2, A. D. Marmorstein^1,2, J. W. Crabb^1,2; ¹Cleveland Clinic Foundation, Cleveland, OH, United States, ²Lerner Research Institute, Cleveland, OH, United States, ³University Medicine Dentistry of New Jersey, Newark, NJ, United States, ⁴University of North Dakota, Grand Forks, ND, United States.
            
            
          • Poster P171-T The National Cancer Institute's Core Genotyping Facility (CGF): Approaching a Gold Standard in Genotyping Assay Validation R. A. Welch , M. Yeager, B. Staats, M. Beerman, B. Packer, A. Hutchinson, A. Bergen, T. Bandey, S. Chowdhury, A. Crenshaw, S. Yadavalli, H. Sicotte, E. Miller, M. Kiley, S. Chanock; NCI/ATC, Gaithersburg, MD, United States.
            
            
          • Poster P179-Th Current Trends in the Resource Facility Providing Genetic Variability Analysis. D. A. Bintzler , P. S. Adams , Y. Bao , R. Keefe , L. O. Petukhova , C. Rosato, R. Scholl , K. Sol-Church.
            
            
          • PosterR14-W ABRF Web Site Committee 2003 Study: Improvements and Demographics of the ABRF Web Site. J. Van Ee ,P. S. Adams , R. H. Lyons , K. I. Mitchelhill , M. Pamukcu , L. Steinke , T. W. Thannhauser, C. D. Walls.
            
            

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      ABRF '02: Biomolecular Technologies: Tools for Discovery in Proteomics and Genomics, March 9-12, 2002, Austin, Texas
      
      
      • Archived meeting information from FASEB site
        
        
      • Abstracts Reprinted with permission of the JBT.
        
        
      • Presentations from ABRF 2002
        • RRF to ABRF to .... : A look back at the history of the ABRF.
          Ronald L. Niece, Research Resources & Technologies
          
          
        • Tutorials
          • Tutorial Session 1: Light Scattering GRAND BALLROOM B Chair: Ewa Folta-Stogniew, Yale Univ., New Haven, CT
            
            T1 Determination of molecular masses of proteins in solution: Implementation of an HPLC size exclusion chromatography and laser light scattering service in a core laboratory.
            Ewa Folta-Stogniew, Yale Univ., New Haven, CT
            
            
          • Tutorial Session 4: Real Time PCR GRAND BALLROOM A Chair: Pamela Scott Adams, Trudeau Inst., Saranac Lake, NY
            
            T7 The importance of RNA quality in Real Time Quantitative RT-PCR.
            Eric Lader, Ambion, Austin,TX
            
            T8 Quantitative real-time RT-PCR - A very short course. Gregory L. Shipley, Univ. of Texas-Houston Med. Sch., Houston, TX
            
            
          • Tutorial Session 5: Synthesis of Real Time PCR Probes GRAND BALLROOM A Chair: Anthony Yeung, Fox Chase Cancer Ctr., Philadelphia,PA
            
            T9 Practical approaches to the synthesis, purification and characterization of dual-labeled DNA probes. Brian Holloway, Biotechnology Core Facility, Atlanta,GA
            
            T10 Preparation and analysis of black hole quencher probes. Joe Knecht, Biosearch Technologies, Inc., Novato,CA
            
            T11 The mechanistic side of fluorescence quenching: a discussion of specialized quenching to enable multiplexing. Cynthia Potter, Biosearch Technologies, Inc., Novato, CA
            
            
        • Roundtable Sessions
          • Roundtable Session R3: Panel/roundtable discussion on service related and technical issues concerning real time PCR.
            Chair: Pamela Scott Adams, Trudeau Inst., Saranac Lake, NY.
            
            
        • Posters
          • Poster P27T DNA Sequencing in a Core Facility: Engineering the Process 3, A Next Generation LIMS System to Integrate Operational Steps in a Large Sequencing Core. J. VanEe, A. Mannochia, T. Thannhauser, T. Stelick, R. Halpin; Cornell University

       

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      ABRF '01: The New Biology: Technology for resolving Macromolecular Communications, February 24-27, 2001, San Diego, CA
      • Archived information from FASEB site
        
        
      • Abstracts (JBT archive)
        
        
      ABRF '00: From Singular to Global Analyses of Biological Systems, Bellvue, WA
      • Preliminary program archived on FASEB Site
      • Abstracts and other info (JBT Archive)

       

      ABRF'99: Bioinformatics and Biomolecular Technologies: Linking Genomes, Proteomes and Biochemistry,March 19 - 22, 1999, Durham, NC
      • Archived information from FASEB site
      • preliminary program (JBT archive)

       

      ABRF'98: From Genomes to Function - Technical Challenges of the Post-Genome Era, March 21-24, 1998, San Diego, CA
      • Archived information from FASEB site
      • Full Program and Abstracts
      • Presentations from ABRF '98

       

      ABRF 97: Techniques at the Genome-Proteome Interface, Baltimore, MD
      • Program (ABRF News archive)
      • Tutorial Notes - Interpreting Automated DNA Sequences and Sequencer Problems, Baltimore 1997

       

      ABRF 96 Biomolecular Techniques in San Francisco, March 30-April 2, 1996
      • Methods for Capillary HPLC Isolation and Collection of Peptides
      • Characterization of Glycosylated proteins in Gels and Blots
      • Tutorial Notes
        • Amino Acid Analysis
        • Interpreting Automated DNA Sequencing
        • Analysis Of Carbohydrates Of Glycoproteins
        • Sequencing Difficult Templates
        • Efficient Electroblotting And On-Membrane Proteolysis at High Sensitivity for Sequence Analysis

       

      ABRF Workshop at Davos, 28 May 1995
      • Scientific Presentations
      • Real-time PCR in Cancer Research
        Genomic Analysis of Ovarian Cancer.
        David Ginzinger, UCSF, San Francisco, CA
        
        
      • Research Group Presentations
      • Nucleic Acids Research Group
        Real-time PCR Survey.
        Brian Holloway, CDC, Atlanta, GA and Tony Yeung, Fox Chase Cancer Center, Philadelphia, PA.
        
      • Fragment Analysis Research Group
        FARG Study 2004: Whodunit? Analysis and Comparison of Equipment and Protocols used for Fragment Analysis
        Caprice Rosato, Oregon State University, Corvallis, OR.
        
        
      • Tutorials
      • DNA Sequencing Research Group
        Quality Control and Crowd Control in the DNA Sequencing Core
        Robert Lyons, University of Michigan, Ann Arbor, MI
        
        
      • Round Table Presentations
      • CEL I mismatch-specific endonuclease A. T. Yeung, E. Nicolas, L. Hancock; Fox Chase Cancer Center, Philadelphia, PA, United States.
        
        
      • Posters
      • R3-S DSRG-2004 - A Web-based User-Interactive DNA Sequencing Troubleshooting Resource. Heather Lin¹, Helaman Escobar², John W. Hawes³, David S. Needleman⁴, Timothy Hunter⁵, Emily Jackson-Machelski⁶, Kevin L. Knudtson⁷, Rashmi Pershad⁸, Margaret Robertson⁹, and Glenis J. Wiebe¹⁰; ¹University of Utah, Salt Lake City, UT, ²Brigham Young University, Provo, UT, ³Indiana University School of Medicine, Indianapolis, IN, ⁴Thomas Jefferson University, Philadelphia, PA, ⁵Vermont Cancer Center, Burlington, VT, ⁶Washington University School of Medicine, St. Louis, MO, ⁷University of Iowa, Iowa City, IA, ⁸University of Texas M.D. Anderson Cancer Center, Houston, TX, ⁹Ernest Galle Clinic and Research Center, UCSF, San Francisco, CA, ¹⁰Max Planck Institute of Molecular Cell Biology & Genetics, Dresden, Germany
        
        
      • R3-S Proteomics Research Group Study 2004: Differentiation of Protein Isoforms. D. Arnott¹, M. A. Gawinowicz², J. A. Kowalak³, W. S. Lane⁴, T. A. Neubert⁵, K. D. Speicher⁶, C. W. Turck⁷, K. A. West⁸; ¹Genentech, Inc., South San Francisco, CA, USA, ²Columbia University, New York, NY, USA, ³NIH, Bethesda, MD, USA, ⁴Harvard University, Cambridge, MA, USA, ⁵New York University School of Medicine, New York City, NY, USA, ⁶The Wistar Institute, Philadelphia, PA, United States, ⁷Max Planck Institute of Psychiatry, Munich, Germany, ⁸Cleveland Clinic Foundation - Cole Eye Institute, Cleveland, OH, USA, .
        
        
      • Poster R6-S Nucleic Acids Research Group 2004 Study: Real-Time PCR Survey. PS Adams¹, Y Bao², DS Grove³,BP Holloway⁴, GL Shipley⁵ and AT Yeung⁶; ¹Trudeau Institute, Saranac Lake, NY, USA, ²University of Virginia, Charlottesville, VA, USA, ³ Pennsylvania State University, University Park, PA, USA, ⁴CDC, Atlanta, GA, USA,⁵University of Texas Health Science Center, Houston, TX, USA,⁶ Fox Chase Cancer Center, Philadelphia, PA, USA
        
        
      • Poster R9-S FARG Study 2004: Whodunit? Analysis and Comparison of Equipment and Protocols used for Fragment Analysis Caprice Rosato¹, Yongde Bao², Doug Bintzler³, Stephen A. Bobin⁴, Joe Forrester⁵, Robert Keefe⁶, Rebecca Scholl⁷, Katia Sol-Church⁸, Tom Stelick⁹; ¹Oregon State University, Corvallis, OR, USA, ²University of Virginia, Charlottesville, VA, USA, ³ University of Cincinnati, Cincinnati, OH, USA, ⁴Dartmouth College, Hanover, NH, USA, ⁵University of Missouri, Columbia, MO, USA, ⁶ Wadsworth Center, NYS DOH, Albany, NY, USA, ⁷University of Utah, Salt Lake City, UT, USA, ⁸Alfred I duPont Hospital for Children, Wilmington, DE, USA, ⁹ Cornell University, Ithaca, NY, USA
        
        
      • P44-M Analysis of Low-Abundance Serum Proteins Using Mass Spectrometry. K. Merrell, C. D. Thulin, K. Southwick, N. E. Lewis; Brigham Young University, Provo, UT, USA.
        
        
      • Poster P130-S Immunoaffinity-Purification and Identification of Teleost Thy-1. S. Deininger¹, M. Przybylski², A. Reuter³, C. A. Stuermer³; ¹Bruker Daltonik GmbH, Leipzig, Germany, ²Department of Chemistry, University of Konstanz, Konstanz, Germany,³Department of Biology, Neurobiology, University of Konstanz, Konstanz, Germany.
        
        
      • Poster 176-M Comparing Microarray Data Generated in Different Laboratories. LH Reid¹, W Jones¹, D Cox¹,AI Brooks²; ¹Expression Analysis, Durham, NC, USA, ²University of Rochester Medical Center, Rochester, NY, USA.