Date: Mon, 12 May 2008 18:23:56 -0400
From: "S Chauhan" <sechau1@yahoo.com>
To: <ABRF@list.abrf.org>
Subject: Using HPLC to calculate protein conc in cell culture.Hi Guys, I am thinking of using HPLC (protein G) for screening of cell culture for a protein (e = 175000 M-1 cm-1). Could you please suggest what kind of column (analytical, semi-analytical) should I use? Also, what is the minimum conc. (micrograms?) that one could see in HPLC? Thanks in advance. Schau
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