Hello all,
I need to do a tryptic digestion from an agarose gel.  The protein is
quite large, and will be the focus of a cross-linking experiment.
The goal is to identify the binding partners of our protein of
interest, and, if possible, identify the site of cross-linking on our
protein.  I will be performing mass spectroscopy, including de novo
sequencing on selected peptides to determine the sequences of
interest.

I have not performed tryptic digestion from agarose.  The protein is
in standard SDS sample buffer.  Do any of you have any hints for me
before we start?

All the best,
-laurey

Weather inconsequential:  Lovely and clear today.  My lilacs are in
full bloom, and I am eating asparagus from my patch, and morels
picked for me on the farm by a cousin----what am I doing in here
worrying about mass spectrometry?
--
Laurey Steinke, Ph.D
Assistant Professor
Department of Biochemistry and Molecular Biology
Protein Structure Core Facility
Mass Spectrometry & Proteomics Facility
985819 Nebraska Medical Center
Omaha, NE  68198-5819
T:  (402) 559-5176
F:  (402) 559-6646
http://www.unmc.edu/pscf/