| Protein Expression (PERG) |
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Current Members |
Studies |
Activities |
Membership History |
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| The mission of the Protein Expression Research Group (PERG) is (1) to educate ABRF members and scientific community on best practices in recombinant protein expression and purification, and (2) help to advance the technology in recombinant protein expression and purification as a public service for laboratories engaged in protein expression and purification.
Scientific areas include eukaryotic expression (baculovirus/insect cells, and mammalian), bacterial expression, cell-free expression, construct design, protein purification (tags, increasing throughput, increasing ease), protein refolding, and cell culture. |
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| Current Membership |
Dr. John W. Hawes
(Chair) - Miami University
Dr. James W. Bryson
- Bristol-Myers Squibb Co.
Preston Hensley
- Pfizer Inc
Dr. Cynthia Kinsland
- Cornell University
Rebecca Page
- Brown University
Francis Rajamohan
- Pfizer Inc.
Dr. Bo Xu
- UTMB
Dr. Arnold M. Falick
(EB liaison) - HHMI-UC Berkeley
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| Studies |
| 1) |
PERG Survey 2007 (Completed). Slides are available summarizing the survey conducted by the ABRF Protein Expression Research Group, February-March 2007. These slides were presented at the Protein Expression Research Group presentation at ABRF 2007 at the Tampa Convention Center, Tampa, Florida.
- PERG_Survey2007_Slides
(18K)
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| Activities |
| 1) |
State of the art technologies for producing high-quality recombinant proteins in core facilities
- Flyer
(171K)
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| 2) |
Jim Bryson: "Design of protein constructs for optimal expression and efficient methods for generating DNA expression vectors
- Jim Bryson
(5,187K)
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| 3) |
Rebecca Page: "Optimization and increasing throughput of expression in E. coli"
- Rebecca Page
(9,457K)
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| 4) |
Francis Rajamohan: "Expression, optimization and production of recombinant proteins in insect cells using baculovirus"
- Francis Rajamohan
(3,514K)
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| 5) |
David Wasilko: "Titerless infected insect cell preservation and scale-up (TIPS)"
- David Wasilko
(1,028K)
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| 6) |
Frederic Boyce: "Baculovirus-mediated gene transfer into mammalian cells"
- Frederic Boyce
(2,763K)
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| 7) |
Yaeto Endo: "Cell-Free recombinant protein expression"
- Yaeto Endo
(17,691K)
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| 8) |
John Hawes: "Characterization of recombinant protein reagent to assure high quality"
- John Hawes
(454K)
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| 9) |
PERG Survey: "Bottlenecks in protein expression" 2007
- PERG Survey
(102K)
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| Membership History |
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