created: 8th November, 2000, last updated: 8th November, 2000 ,© 2000 ABRF
The ABRF Peptide Synthesis Research Group would like to invite member laboratories to participate in the 2001 study. This year’s study is designed to provide an updated examination of improvements to peptide synthesis chemistry, in particular, those changes that facilitate synthesis of difficult sequences. Peptide synthesis labs are continually challenged with peptides that are often challenging at best. Synthesis of such difficult peptides can yield little or no desired product. Even if the desired product is present, purification can be just as challenging. Development of improved methodologies, synthesis reagents, and amino acid analogs may potentially offer new solutions for the old problem of difficult sequences. The PSRG would like to compare synthesis schemes and purification strategies that different member laboratories employ to improve the quality and separation of the crude peptide. Analysis performed by the PSRG will include analytical HPLC, AAA, MALDI-MS, ESI-MS.
The PSRG would like participating labs to synthesize the following peptide:
NH2-QTSSGTTSWVTSRRDGAGAGP-OH
The peptide was designed to encompass a number of side reactions and difficult stretches. For example, the C-terminal Gly-Pro, if synthesized using a standard Wang resin will likely result in diketopiperazine formation, resulting in little to no yield. A number of alternative resins have been developed which can be used which prevent diketopiperazine formation.
In addition to comparing synthesis strategies with the crude product, the PSRG would also like to determine success rates in purifying peptides from a heterogeneous mixture. To accomplish this, we would like participating labs to also purify a portion of the peptide that will then be sent to the PSRG for analysis along with the crude material. We ask you to also note the amount of crude peptide you began purification with and the final yield to obtain a percentage of recovered, purified peptide.
Download the Materials and Methods Form
Please send five 1mg aliquots of crude and five 1mg aliquots of purified material, and the materials and methods form to:
Ms. Christy Sasiela
University of Maryland School of Pharmacy
20 North Pine Street, Room 500
Baltimore, Maryland 21201
410-706-2961
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