AAA Performance Overview 1995

Sample Categories: Experimental vs Standards/Controls

Sample

Amino Acid Standards

e.g., Pierce Standard H

Peptide and Protein Standards

e.g., Applied Biosystems Hydrolysis Test Peptide
National Bureau of Standards BSA

Blanks

Test Methods

e.g., Cys, Trp, etc

Experimental samples

 % of Total Analyses

29


14



9

6


42

For PTC-AAA (n=4632 analyses) at the W. Alton Jones Cell Science Center

Approximate Labor Requirements for AAA

Activity

Manual Hydrolysis

set up, drying, dissolving

Loading Samples

Instrument Calibration

checking separation, reprocessing data,
adjusting response factors

Data Reduction/Experimental samples

Instrument maintenance

Making Buffers, Service, and Bookwork

 Approximate Time needed

~1 hr / 5-20 samples


~15 mins/ 5-20 samples

~45 mins/ 6 standards



~30 mins/ 5-10 samples

~30 mins/ 20-50 samples

Based on our experience with the Applied Biosystem Model 420H/920/130 System

PTC-AAA Cost Overview

Items

Supplies

57% general
38% HPLC, Solvents, Columns
3% Argon and Nitrogen gas
2% Computer paper / discs

Service Contract

Labor

Total Approximate cost/analysis

 cost/analysis

$9.65





$3.35

$24.00

$37.00

Based on 4632 analysis with the Applied Biosystems Models 420H/920/130 System

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